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Biochemistry

Head

  • Charles M. Brenner

Professors

  • Charles M. Brenner, Pamela Geyer, George Giudice, David H. Price, Peter Rubenstein, Madeline A. Shea, Lori L. Wallrath, Daniel L. Weeks, Ronald Weigel, Marc S. Wold

Professors emeriti

  • Arthur Arnone, Thomas W. Conway, John Donelson, Alice B. Fulton, Rex Montgomery, Bryce Plapp, Arthur A. Spector, Earle Stellwagen, Charles A. Swenson

Adjunct professors

  • Theresa Gioannini, Nancy C. Stellwagen, Ramaswamy Subramanian, Joseph Walder, Liping Yu

Associate professors

  • John Dagle, Adrian Elcock, Maria Spies, M. Todd Washington

Assistant professors

  • Sheila A. Baker, Heather L. Bartlett, Kris DeMali, Ernesto Fuentes, Miles Pufall, M. Ashley Spies

Adjunct assistant professor

  • Lokesh Gahkar

Lecturer

  • Elisabeth Swain
Undergraduate major: biochemistry (B.A., B.S.)
Graduate degrees: M.S. in biochemistry; Ph.D. in biochemistry
Web site: http://www.biochem.uiowa.edu/

Biochemistry is the study of basic chemical processes that occur in and govern all living systems. Nearly all areas of the life sciences engage in biochemical research.

Biochemistry graduates with bachelor's degrees often work as research assistants in industry, government, education, or health services; teach in secondary schools; or go on to advanced study in medicine, dentistry, or other areas. Those with advanced degrees pursue careers as teachers, researchers, or administrators in universities and medical schools, government, research agencies, and varied industries.

The Department of Biochemistry offers an undergraduate major and graduate degree programs and determines the curricula for those programs. Undergraduates majoring in biochemistry receive their degrees (B.A. or B.S.) from the College of Liberal Arts and Sciences, and their studies are governed by that college's undergraduate academic policies. Graduate degrees in biochemistry are conferred by the Graduate College.

Undergraduate Programs

  • Major in biochemistry (Bachelor of Science, Bachelor of Arts)

Bachelor of Science

The Bachelor of Science with a major in biochemistry requires a total of 120 s.h., including 73 s.h. of work for the major. In order to count transfer credit in biochemistry toward the major, students must have the approval of an undergraduate advisor in the department.

The biochemistry major for the B.S. prepares students to work in positions that require a mastery of general biochemistry. It is also excellent preparation for graduate study in biochemistry and related sciences or for study toward a professional degree in the health sciences.

Students are encouraged to take courses from other disciplines, such as business, pre-law, psychology, or journalism. This prepares them for a variety of career paths.

All students must complete the College of Liberal Arts and Sciences General Education Program.

The biochemistry major for the Bachelor of Science requires the following course work.

All of these:

004:011 (CHEM:1110)-004:012 (CHEM:1120) Principles of Chemistry I-II8 s.h.
22M:025 (MATH:1850)-22M:026 (MATH:1860) Calculus I-II10 s.h.
029:081 (PHYS:1611)-029:082 (PHYS:1612) Introductory Physics I-II8 s.h.
099:101 (BIOC:3101) Technical Communication in Biochemistry1 s.h.
099:120 (BIOC:3120) Biochemistry and Molecular Biology I3 s.h.
099:130 (BIOC:3130) Biochemistry and Molecular Biology II3 s.h.
099:140 (BIOC:3140) Experimental Biochemistry2 s.h.
Advanced science electives, chosen in consultation with advisor9 s.h.

One of these:

099:155 (BIOC:4155) Research, Independent Study6 s.h.
Lab-intensive advanced science electives, chosen in consultation with advisor6 s.h.

One of these sequences:

002:010 (BIOL:1402)-002:011 (BIOL:1502) Principles of Biology I-II (not offered after fall 2012)8 s.h.
002:031 (BIOL:1411)-002:032 (BIOL:1412) Foundations of Biology - Diversity of Form and Function8 s.h.

Courses 002:010 (BIOL:1402) and 002:011 (BIOL:1502) will not be offered after fall semester 2012, so students who satisfy the biology requirement with those courses must complete them by the end of that semester.

One of these sequences:

004:123 (CHEM:2230)-004:124 (CHEM:2240) Organic Chemistry I for Majors - Organic Chemistry II for Majors (recommended)6 s.h.
004:121 (CHEM:2210)-004:122 (CHEM:2220) Organic Chemistry I-II (accepted)6 s.h.

One of these:

004:141 (CHEM:2410) Organic Chemistry Laboratory3 s.h.
004:142 (CHEM:2420) Organic Chemistry Laboratory for Majors3 s.h.

Two of these:

004:131 (CHEM:4431) Physical Chemistry I3 s.h.
004:132 (CHEM:4432) Physical Chemistry II3 s.h.
099:241 (BIOC:5241) Biophysical Chemistry I3 s.h.
099:242 (BIOC:5242) Biophysical Chemistry II3 s.h.

Students are encouraged to begin research by taking 099:115 (BIOC:3115) Undergraduate Independent Study, which has no prerequisites. The course involves experience in an active biochemistry research lab, which must be arranged ahead of time with a supervising faculty member. Students may make arrangements directly with the faculty member or they may request assistance from an undergraduate advisor. Credit earned in 099:115 (BIOC:3115) does not count toward the major, but it does count toward the minimum 120 s.h. required to graduate.

In order to register for 099:155 (BIOC:4155) Research, Independent Study, students must have a completed 099:120 (BIOC:3120), 099:130 (BIOC:3130), and 099:140 (BIOC:3140) with a grade average of B or higher in the three courses, and they must not have earned a grade below B-minus in any one of them; or they must have their advisor's consent and the instructor's consent to enroll in 099:155 (BIOC:4155).

Bachelor of Arts

The Bachelor of Arts in biochemistry requires a total of 120 s.h., including 57 s.h. of work for the major. In order to count transfer credit in biochemistry toward the major, students must have the approval of an undergraduate advisor in the department.

Students are encouraged to take courses from other disciplines, such as business, pre-law, psychology, or journalism. This prepares them for a variety of career paths.

All students must complete the College of Liberal Arts and Sciences General Education Program.

The biochemistry major for the Bachelor of Arts requires the following course work.

All of these:

004:011 (CHEM:1110)-004:012 (CHEM:1120) Principles of Chemistry I-II8 s.h.
22M:025 (MATH:1850)-22M:026 (MATH:1860) Calculus I-II10 s.h.
029:011 (PHYS:1511)-029:012 (PHYS:1512) College Physics I-II8 s.h.
099:120 (BIOC:3120) Biochemistry and Molecular Biology I3 s.h.
099:130 (BIOC:3130) Biochemistry and Molecular Biology II3 s.h.
099:140 (BIOC:3140) Experimental Biochemistry2 s.h.
Advanced science electives, chosen in consultation with advisor6 s.h.

One of these sequences:

002:010 (BIOL:1402)-002:011 (BIOL:1502) Principles of Biology I-II (not offered after fall 2012)8 s.h.
002:031 (BIOL:1411)-002:032 (BIOL:1412) Foundations of Biology - Diversity of Form and Function8 s.h.

Courses 002:010 (BIOL:1402) and 002:011 (BIOL:1502) will not be offered after fall semester 2012, so students who satisfy the biology requirement with those courses must complete them by the end of that semester.

One of these sequences:

004:123 (CHEM:2230)-004:124 (CHEM:2240) Organic Chemistry I for Majors - Organic Chemistry II for Majors (recommended)6 s.h.
004:121 (CHEM:2210)-004:122 (CHEM:2220) Organic Chemistry I-II (accepted)6 s.h.

One of these:

004:131 (CHEM:4431) Physical Chemistry I3 s.h.
004:132 (CHEM:4432) Physical Chemistry II3 s.h.
099:241 (BIOC:5241) Biophysical Chemistry I3 s.h.
099:242 (BIOC:5242) Biophysical Chemistry II3 s.h.

Bachelor of Arts students who intend to go on to advanced degrees in the biological or health sciences are advised to earn at least 4 s.h. in 099:115 (BIOC:3115) Undergraduate Independent Study or 099:155 (BIOC:4155) Research, Independent Study. There are no prerequisites for 099:115 (BIOC:3115). The course involves experience in an active biochemistry research lab, which must be arranged ahead of time with a supervising faculty member. Students may make arrangements directly with the faculty member or they may request assistance from an undergraduate advisor. Credit earned in 099:115 (BIOC:3115) does not count toward the major, but it does count toward the minimum 120 s.h. required to graduate.

In order to register for 099:155 (BIOC:4155) Research, Independent Study, students must have completed 099:120 (BIOC:3120), 099:130 (BIOC:3130), and 099:140 (BIOC:3140) with a grade average of B or higher in the three courses, and they must not have earned a grade below B-minus in any one of them; or they must have their advisor's consent and the instructor's consent to enroll in 099:155 (BIOC:4155).

B.A. or B.S. with Teacher Licensure

Biochemistry majors interested in earning licensure to teach in elementary and/or secondary schools must complete the College of Education's Teacher Education Program (TEP) in addition to the requirements for the major and all requirements for graduation. The TEP requires several College of Education courses and student teaching. Contact the Office of Education Services for details.

Students must satisfy all degree requirements and complete Teacher Education Program licensure before degree conferral.

Four-Year Graduation Plan

The following checkpoints list the minimum requirements students must complete by certain semesters in order to stay on the University's Four-Year Graduation Plan. (Courses in the major are those required to complete the major; they may be offered by departments other than the major department.)

Bachelor of Science

Before the third semester begins: 004:011 (CHEM:1110) Principles of Chemistry I and 004:012 (CHEM:1120) Principles of Chemistry II, 22M:025 (MATH:1850) Calculus I and 22M:026 (MATH:1860) Calculus II, and at least one-quarter of the semester hours required for graduation

Before the fifth semester begins: the courses listed above, plus 002:031 (BIOL:1411) Foundations of Biology and 002:032 (BIOL:1412) Diversity of Form and Function; 004:121 (CHEM:2210) Organic Chemistry I, 004:122 (CHEM:2220) Organic Chemistry II, and 004:141 (CHEM:2410) Organic Chemistry Laboratory; and at least one-half of the semester hours required for graduation

Before the seventh semester begins: the courses listed above, plus 029:081 (PHYS:1611) Introductory Physics I and 029:082 (PHYS:1612) Introductory Physics II, 099:120 (BIOC:3120) Biochemistry and Molecular Biology I, 099:130 (BIOC:3130) Biochemistry and Molecular Biology II, 099:101 (BIOC:3101) Technical Communication in Biochemistry, and 099:140 (BIOC:3140) Experimental Biochemistry, two science electives, and at least three-quarters of the semester hours required for graduation

Before the eighth semester begins: the courses listed above, plus 004:131 (CHEM:4431) Physical Chemistry I or 004:132 (CHEM:4432) Physical Chemistry II or 099:241 (BIOC:5241) Biophysical Chemistry I or 099:242 (BIOC:5242) Biophysical Chemistry II, a science elective, and at least 3 s.h. of 099:155 (BIOC:4155) Research, Independent Study

During the eighth semester: enrollment in all remaining course work in the major, all remaining General Education courses, and a sufficient number of semester hours to graduate

Bachelor of Arts

Before the third semester begins: 004:011 (CHEM:1110) Principles of Chemistry I and 004:012 (CHEM:1120) Principles of Chemistry II; math through 22M:026 (MATH:1860) Calculus II or above; and at least one-quarter of the semester hours required for graduation

Before the fifth semester begins: the courses listed above, plus 002:031 (BIOL:1411) Foundations of Biology and 002:032 (BIOL:1412) Diversity of Form and Function, 004:121 (CHEM:2210) Organic Chemistry I and 004:122 (CHEM:2220) Organic Chemistry II, and at least one-half of the semester hours required for graduation

Before the seventh semester begins: the courses listed above, plus 029:011 (PHYS:1511) College Physics I and 029:012 (PHYS:1512) College Physics II; 099:120 (BIOC:3120) Biochemistry and Molecular Biology I, 099:130 (BIOC:3130) Biochemistry and Molecular Biology II, and 099:140 (BIOC:3140) Experimental Biochemistry; two science electives, and at least three-quarters of the semester hours required for graduation

Before the eighth semester begins: the courses listed above, plus 004:131 (CHEM:4431) Physical Chemistry I or  004:132 (CHEM:4432) Physical Chemistry II or 099:241 (BIOC:5241) Biophysical Chemistry I or 099:242 (BIOC:5242) Biophysical Chemistry II, and a science elective

During the eighth semester: enrollment in all remaining course work in the major, all remaining General Education courses, and a sufficient number of semester hours to graduate

Honors

Qualified students may work toward graduation with honors in biochemistry. They must be members of the University of Iowa Honors Program, which requires that students maintain a cumulative University of Iowa g.p.a. of at least 3.33 (contact the University of Iowa Honors Program for more information). To graduate with honors in the major, students must earn 6 s.h. in 099:155 (BIOC:4155) Research, Independent Study. They must present their research results in a report written in the form of a journal article and in an oral report given at a special open departmental seminar.

Graduate Programs

  • Master of Science in biochemistry
  • Doctor of Philosophy in biochemistry

Students admitted to graduate study in biochemistry usually pursue the Doctor of Philosophy. Qualified students interested in earning the Doctor of Medicine along with the Ph.D. may apply to the Medical Scientist Training Program, which offers a joint M.D./Ph.D. program.

Master of Science

The Master of Science program in biochemistry requires a minimum of 30 s.h. of graduate credit, thesis research, and a thesis. See "Doctor of Philosophy" for information about the graduate curriculum.

Doctor of Philosophy

The Doctor of Philosophy program in biochemistry requires a minimum of 72 s.h. of graduate credit. The focus of the graduate program is on the individual student.

The 72 s.h. required for the degree includes 34 s.h. of course work and 38 s.h. of research credit. Students may take courses that enhance their educational goals. All Ph.D. students take the following 19 s.h. of course work; they choose an additional 13 s.h. from courses offered by the Department of Biochemistry and other University of Iowa departments.

099:261 (BIOC:5261) Research Techniques (first-year laboratory rotation)1-5 s.h.
650:270 (GRAD:7270) Principles of Scholarly Integrity0-1 s.h.
Biophysical chemistry (students typically earn 6 s.h.)3 s.h.
Molecular or cellular biology (students typically earn 6-8 s.h.)4 s.h.
Graduate seminar3 s.h.

The following is a typical first-year curriculum.

First semester:

099:243 (BIOC:5243) Biophysical Chemistry Module 1: Protein Structure, Stability, and Dynamics1 s.h.
099:245 (BIOC:5245) Biophysical Chemistry Module 2: Structure and Stability of Proteins and Nucleic Acids1 s.h.
099:247 (BIOC:5247) Biophysical Chemistry Module 3: Biophysics of Macromolecular Interactions1 s.h.
099:261 (BIOC:5261) Research Techniques1-5 s.h.
156:201 (BISC:5201) Fundamentals of Gene Expression1 s.h.
156:203 (BISC:5203) Fundamentals of Dynamic Cell Processes1 s.h.
156:204 (BISC:5204) Biostatistics for Biomedical Research1 s.h.
156:265 (BISC:5265) Biosciences Critical Thinking and Communication2 s.h.
650:270 (GRAD:7270) Principles of Scholarly Integrity0-1 s.h.

Second semester:

099:226 (BIOC:5226) Enzyme Kinetics and Bioorganic Mechanisms1-2 s.h.
099:244 (BIOC:5244) Biophysical Chemistry Module 4: Ligand Binding and X-Ray Crystallography1 s.h.
099:246 (BIOC:5246) Biophysical Chemistry Module 5: Enzyme Kinetics and Enzyme Mechanisms1 s.h.
099:248 (BIOC:5248) Biophysical Chemistry Module 6: Nuclear Magnetic Resonance Spectroscopy1 s.h.
099:261 (BIOC:5261) Research Techniques1-5 s.h.
142:215 (MCB:6215) Transcription and Multi-Functional Regulation by RNA1 s.h.
142:216 (MCB:6216) Chromatin Structure and Disease1 s.h.
142:217 (MCB:6217) Cancer, Epigenetics, and Genetic Manipulations in Mice1 s.h.
156:205 (BISC:5205) Practical Bioinformatics1 s.h.
156:265 (BISC:5265) Biosciences Critical Thinking and Communication2 s.h.

099:282 (BIOC:5282) Seminar0-1 s.h.
or
156:265 (BISC:5265) Biosciences Critical Thinking and Communication2 s.h.

Once students are promoted to the second year of study, they choose research laboratories for Ph.D. thesis research and begin their thesis projects. They take courses that supplement their interests and preparation, including the following.

099:282 (BIOC:5282) Seminar1 s.h.
099:292 (BIOC:7292) Research Biochemistryarr.
Graduate-level science electives6 s.h.

Students take the comprehensive examination before the end of June in their second year, after which they are admitted formally to degree candidacy and begin to concentrate on thesis research. The program culminates in successful defense of completed thesis work before an examining committee.

In addition to meeting these requirements and those of the Graduate College, students are expected, as part of their training, to assist in teaching biochemistry for one semester.

Throughout the program, students are associated with faculty-directed research groups. They receive close personal attention from the biochemistry faculty members who serve as research advisors.

Admission

Most graduate students in biochemistry work toward a Doctor of Philosophy. Applicants who wish to earn a master's degree must contact the Department of Biochemistry before they apply.

The graduate program in biochemistry is flexible enough to accommodate students with bachelor's degrees in any of the biological, biochemical, or physical sciences. Appropriate preparation includes one-year, college-level courses in organic and physical chemistry, biology, physics, and mathematics through calculus. Students are expected to have had one or more introductory courses in biochemistry.

Applicants must have an undergraduate g.p.a. of at least 3.00 and must submit acceptable verbal, quantitative, and analytical scores on the Graduate Record Examination (GRE) General Test. Applicants are encouraged to submit their scores on the GRE Subject Test in Chemistry; Biology; or Biochemistry, Cell, and Molecular Biology.

Applicants must meet the admission requirements of the Graduate College; see the Manual of Rules and Regulations of the Graduate College or the Graduate College section of the Catalog.

Financial Support

Students admitted to the Ph.D. program in biochemistry routinely receive a stipend and tuition support.

Faculty and Research

The department's faculty members supervise research in biochemistry; molecular, cellular, developmental, computational, and structural biology; and model system genetics. Their work is supported by grants from the National Institutes of Health, the National Science Foundation, the American Heart Association, the American Cancer Society, the Muscular Dystrophy Association, and other sources. To learn more about the department's faculty members and areas of research, visit the Department of Biochemistry web site.

Facilities

The Department of Biochemistry occupies 36,700 square feet on the fourth floor of the Bowen Science Building and 7,500 square feet on the third floor of the Medical Education Research Facility on the University's health sciences campus. It has a number of well-equipped research laboratories; other departmental facilities include the Biochemistry Stores, the Mattill Biochemistry Reading Room, and the Heath Conference Room.

The department makes available a number of shared instruments; among them are an Applied PhotoPhysics stopped flow spectrometer SX20 (2009); a Jasco spectropolarimeter, model J815 (2010); a Horiba fluorlog-3 spectrofluorometer (2010); and a Beckman Coulter ultra XLI analytical centrifuge (1996).

Faculty, staff, and students in the department have access to a variety of shared Carver College of Medicine resources, including X-ray crystallography, the DNA Facility, the NMR Facility, the Proteomics Facility, the Flow Cytometry Facility, the Gene Transfer Vector Core Facility, the Small Animal Imaging Core, and the Transgenic Animal Facility. The University also supports resources such as the Central Microscopy Research Facilities and the Center for Biocatalysis and Bioprocessing.

Courses

099:101 (BIOC:3101) Technical Communication in Biochemistry1 s.h.
Practical aspects of writing formal scientific papers and giving oral presentations on technical topics. Prerequisites: 099:120 (BIOC:3120) or 099:130 (BIOC:3130) or 099:140 (BIOC:3140). Requirements: junior or senior biochemistry major pursuing a B.S. degree. 
 
099:110 (BIOC:3110) Biochemistry3 s.h.
Basic concepts in modern biochemistry and molecular biology; understanding of life processes in molecular terms. Requirements: one year each of college‑level biology and chemistry. Recommendations: one semester of organic chemistry.
 
099:115 (BIOC:3115) Undergraduate Independent Studyarr.
Experience in an active biochemistry research lab, learning and performing experiments relevant to the current projects in that lab; exploration of scientific literature on topic of interest; arranged in advance by student and faculty member. Requirements: first‑year, sophomore, or junior standing.
 
099:120 (BIOC:3120) Biochemistry and Molecular Biology I3 s.h.
Physical and chemical foundations of biochemistry, structure of biological molecules, catalysis, transport, and oxidative reactions in biology; first course of two‑semester sequence that concludes with 099:130 (BIOC:3130). Requirements: two semesters of general chemistry and one of organic chemistry. Recommendations: 002:010 (BIOL:1402) and 002:011 (BIOL:1502), or 002:031 (BIOL:1411) and 002:032 (BIOL:1412); and an additional organic chemistry course.
 
099:130 (BIOC:3130) Biochemistry and Molecular Biology II3 s.h.
Carbohydrate biosynthesis, lipid metabolism, hormone regulation and integration of metabolism, signal transduction, genes and chromosomes, DNA replication and repair, transcription, RNA processing, protein translation and regulation of gene expression. Prerequisites: 099:120 (BIOC:3120).
 
099:140 (BIOC:3140) Experimental Biochemistry2 s.h.
Use of modern instruments and techniques to fractionate, identify, and characterize constituents of biochemical systems. Prerequisites: 099:120 (BIOC:3120). Requirements: grade of C or higher in 099:120 (BIOC:3120), two semesters of general chemistry, and one semester of organic chemistry.
 
099:155 (BIOC:4155) Research, Independent Studyarr.
Independent study and research in areas of interest to the student; arranged in advance by student and biochemistry honors advisor. Requirements: grades of B‑ or higher in 099:120 (BIOC:3120), 099:130 (BIOC:3130), and 099:140 (BIOC:3140); and an average grade of B or higher in all three courses.
 
099:161 (BIOC:8101) Biochemistry for Dental Students4 s.h.
Biochemical concepts and application to clinical problems. Requirements: 004:121 (CHEM:2210), and D.D.S. enrollment. Recommendations: 004:122 (CHEM:2220).
 
099:162 (BIOC:8102) Biochemistry for Pharmacy Students4 s.h.
Biochemical concepts and application to clinical problems. Requirements: 004:121 (CHEM:2210) and Pharm.D. enrollment. Recommendations: 004:122 (CHEM:2220).
 
099:163 (BIOC:8103) Medical Biochemistry4 s.h.
Biochemical concepts and application to clinical problems. Requirements: M.D. enrollment.
 
099:164 (BIOC:8204) Biochemistry for Physician Assistant Students3 s.h.
Aspects of general biochemistry necessary for understanding the biochemical basis of human disease; analysis of appropriate clinical cases. Prerequisites: 099:110 (BIOC:3110).
 
099:215 (BIOC:5215) Directed Readings for Graduate Studentsarr.
Directed readings with course content arranged with professor.
 
099:226 (BIOC:5226) Enzyme Kinetics and Bioorganic Mechanisms1-2 s.h.
Principles and applications of steady‑state and transient enzyme kinetics; mechanisms of catalysis of biochemical reactions. Prerequisites: 099:120 (BIOC:3120).
 
099:238 (BIOC:6238) Topics in Biophysical Chemistry1-2 s.h.
Current topics in structure and function of membranes or proteins; DNA‑protein interactions; computational biochemistry; applications of NMR, X‑ray diffraction, calorimetry, or spectroscopy. Prerequisites: 099:241 (BIOC:5241) or 099:242 (BIOC:5242).
 
099:241 (BIOC:5241) Biophysical Chemistry I3 s.h.
Principles and experimental approaches used to study structure and function of biological macromolecules; protein structure, stability, and dynamics; macromolecular interactions; common biophysical methods. Requirements: one year of biochemistry. Recommendations: physical chemistry course and one semester of calculus.
 
099:242 (BIOC:5242) Biophysical Chemistry II3 s.h.
Principles and experimental approaches used to study structure and function of biological macromolecules; ligand binding and enzyme catalysis; X‑ray crystallography; NMR spectroscopy. Requirements: one year of biochemistry. Recommendations: physical chemistry course and one semester of calculus.
 
099:243 (BIOC:5243) Biophysical Chemistry Module 1: Protein Structure, Stability, and Dynamics1 s.h.
Overview of principles of protein structure, stability, folding, and dynamics; brief treatment of structural biology approaches to help students become critical users of models derived from X‑ray crystallography and NMR; taken alone or as part of 099:241 (BIOC:5241). Requirements: introductory course in biochemistry.
 
099:244 (BIOC:5244) Biophysical Chemistry Module 4: Ligand Binding and X-Ray Crystallography1 s.h.
In‑depth examination of principles of ligand binding; experimental approaches to study interactions with small molecules, proteins, and nucleic acids; X‑ray crystallography in determining structures of biological macromolecules; taken alone or as part of 099:242 (BIOC:5242). Requirements: introductory course in biochemistry.
 
099:245 (BIOC:5245) Biophysical Chemistry Module 2: Structure and Stability of Proteins and Nucleic Acids1 s.h.
In‑depth examination of statistical thermodynamics and molecular forces in biological systems as related to protein structure, stability, and folding; nucleic acid structure and stability; taken alone or as part of 099:241 (BIOC:5241). Requirements: introductory course in biochemistry.
 
099:246 (BIOC:5246) Biophysical Chemistry Module 5: Enzyme Kinetics and Enzyme Mechanisms1 s.h.
In‑depth examination of principles of steady‑state and pre‑steady‑state kinetics; mechanisms of catalysis of a wide range of biochemical reactions; taken alone or as part of 099:242 (BIOC:5242). Requirements: introductory course in biochemistry.
 
099:247 (BIOC:5247) Biophysical Chemistry Module 3: Biophysics of Macromolecular Interactions1 s.h.
In‑depth examination of protein‑protein interactions and protein‑nucleic acid interactions; implications in biological motility, transcription, and replication; taken alone or as part of 099:241 (BIOC:5241). Requirements: introductory course in biochemistry.
 
099:248 (BIOC:5248) Biophysical Chemistry Module 6: Nuclear Magnetic Resonance Spectroscopy1 s.h.
Basic principles of nuclear magnetic resonance (NMR) and applications important for understanding structure and function of biological macromolecules; emphasis on methodology and experimental design, interpretation of data, and critical reading of literature; intended for graduate students with an interest in applications of NMR to problems of structural biology; taken alone or as part of 099:242 (BIOC:5242). Requirements: one year of biochemistry. Recommendations: basic knowledge of spectroscopy and some previous exposure to NMR from basic chemistry courses.
 
099:253 (BIOC:7253) Metabolism I1 s.h.
Basics of carbohydrate metabolism (glycolysis, gluconeogenesis, the pentose phosphate pathway), hormonal regulation of carbohydrate metabolism, the citric acid cycle, amino acid catabolism, oxidative phosphorylation; assignment of an advanced topic related to material, typically a recent research paper, extending inquiry beyond that presented in class and presented orally at end of five‑week module. Requirements: undergraduate biochemistry course or background in enzyme function.
 
099:254 (BIOC:7254) Metabolism II1 s.h.
Central carbon metabolism, carbohydrate biosynthesis in plants and bacteria, lipid structure/function, fatty acid catabolism, lipid biosynthesis, and biological membranes/transport; assignment of an advanced topic related to material, typically a recent research paper, extending inquiry beyond that presented in class and presented orally at end of five‑week module. Prerequisites: 099:253 (BIOC:7253).
 
099:256 (BIOC:7256) Molecular Biology1 s.h.
DNA, RNA, and protein metabolism, regulation of gene expression, and DNA‑based information technologies.
 
099:261 (BIOC:5261) Research Techniques1-5 s.h.
Laboratory rotation for first‑year graduate students in biochemistry.
 
099:275 (BIOC:5875) Perspectives in Biocatalysis1-3 s.h.
Applied enzymology, protein design, structure‑activity relationships, biosensor technology, microbial transformations, biodegradation of environmental pollutants. Requirements: graduate standing in a participating department supported by the Predoctoral Training Program in Biotechnology. Same as 004:275 (CHEM:5875), 046:275 (PHAR:5875), 052:275 (CBE:5875), 053:275 (CEE:5875), 061:275 (MICR:5875).
 
099:282 (BIOC:5282) Seminar0-1 s.h.
How to evaluate reports of scientific investigations critically; techniques for presenting scientific information.
 
099:283 (BIOC:6283) Thesis Seminar1 s.h.
Preparation and oral presentation of thesis proposal. Requirements: second‑year graduate standing in biochemistry.
 
099:292 (BIOC:7292) Research Biochemistryarr.
Thesis research.